The leftmost slider controls the number of extra negative decades you can add. Adjust the transform by using the sliders in the Transform section of the panel. (Optional, for non-log and non-linear scales). Note, for some scale options you will have to use the Transforms section to adjust visualization space towards the left and/or bottom of the Graph Window.ħ. Use the “+” and “-” buttons or the Min and Max field boxes to adjust the range of the selected scale. For details about each scale click here.Ħ. FlowJo v10 offers 7 different scaling options. Select the scale you wish to use by clicking the drop-down menu in the section labeled “Scale”. Select “Customize Axis” from the list and and an interface will open as shown below:ĥ. Open a graph window and select the X or Y parameter you wish to change.Ĥ.
The following steps will guide you through adjusting the scaling range and/or transformation of a parameter.ġ. To specify a scale range, or use advanced scaling transformations, select the “Customize Axis” option at the bottom of the drop-down menu.
The “Advanced Scale Changes” section below will guide you through these options. Use the “Reset” option to change your axis back to its default setting.įor advanced transformations including biexponential, logicle, arcsinh and others, or to input a scale’s range, select the “Customize Axis” option. You can quickly change the scale of any parameter to logarithmic or linear by clicking the “T” button and selecting “Log Axis” or “Linear Axis” from the drop-down menu, respectively. In addition, the T button will appear to be pulsating, with an orange glowing border around the “T”.Īll parameters’ scaling from any cytometer, including ratiometric parameters or derived parameters, can be adjusted by using the “T” button. If you happen to have many events (>8%) “squished” on either the X- or Y-axes, the Graph Window provides you with a warning detailing the number of events that are on any axis. Red arrows indicate positions of events before and after transformation.
Pseudocolor graphs in the upper panel are displayed as contour plots in the lower panel, but otherwise are identical. Note the densely packed events are actually part of three distinct populations near 0. Densely packed events on the left Y-axis and bottom X-axis were resolved by clicking the T button and selecting the biexponential scale and reducing one decade from the right and upper axes. Using the T button to resolve events on the axes.
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